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Advanced Photon Counting

Applications, Methods, Instrumentation

Language EnglishEnglish
Book Hardback
Book Advanced Photon Counting Peter Kapusta
Libristo code: 09191193
Publishers Springer International Publishing AG, April 2015
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing lumi... Full description
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This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a fingerprint for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing interesting new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.§

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About the book

Full name Advanced Photon Counting
Language English
Binding Book - Hardback
Date of issue 2015
Number of pages 370
EAN 9783319156354
ISBN 3319156357
Libristo code 09191193
Weight 712
Dimensions 169 x 247 x 25
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