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Cell-Free Gene Expression

Name: Cell-Free Gene Expression
Brand: Springer-Verlag New York Inc.
SKU: 38499697
Price: 221.19 EUR
Availability: InStock
Author: Ashty S. Karim
ISBN: 9781071619971

Methods and Protocols

Ashty S. Karim

Michael C. Jewett

Language

English

Book Hardback

Libristo code: 38499697

Publishers Springer-Verlag New York Inc., January 2022

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable ne... Full description

Libristo code: 38499697

535 b

221.19 €

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This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.