Programmed Cell Death

This volume contains§protocols specifically designed for studying programmed cell death, and also§discusses recent advances in techniques that span broader areas of biology that§have been recently used or that have potential to be incorporated into cell death§research. The protocols are mostly described in the context of mammalian§systems, but also cover other systems such as plants, Drosophila , and§yeast. Programmed Cell Death: Methods and Protocols is comprised of 20§chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9§describe methods for studying apoptosis associated with various pathologies in§different organs including the lymphoid compartment, intestinal epithelium,§granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques§for studying apoptosis in non-mammalian systems; Chapters 14-16 cover§biochemical and biophysical methods for studying Bcl-2 family protein dynamics§and protein-protein interactions during apoptosis; and the last four chapters§explore protocols that are useful not only in apoptosis research but in wider§areas of biological research, such as genome editing, inducible transgenes, and§proteomics. Written in the highly successful Methods in Molecular Biology§aeries format, chapters include introductions to their respective topics, lists§of the necessary material and reagents, step-by-step, readily reproducible§laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.§§Thorough§and cutting-edge, Programmed Cell Death: Methods and Protocols is a§comprehensive and valuable resource for researchers, ranging from beginner to§expert, in their studies on programmed cell death.§§