Nitric Oxide Synthase: Characterization and Functional Analysis

Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field. It includes comprehensive protocols for the detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques. It contains newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression. It provides an assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone. It also includes calcium imaging by confocal microscopy, and evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and - 2 message level and distribution.